Primer3 is a command line tool to select primers for polymerase chain reaction (PCR).
All source code of the crispor.org website
Clone with Python! Data structures for double stranded DNA & simulation of homologous recombination, Gibson assembly, cut & paste cloning.
An Open-Source web application to plan and document cloning and genome engineering
Fast DNA search and [host] depletion using minimizers
Terminal-based plasmid map viewer, sequence editor, and cloning/mutagenesis workbench in pure Python
Simple oligo analysis and primer design